The sustainable urbanization project of the New Urban Port District (DUP) at the Port of Seville provides an opportunity to demonstrate innovative mobility solutions. In collaboration with CTAG, this proof-of-concept (PoC) will showcase the potential of autonomous and connected shuttles operating in real-world, high-demand scenarios. The autonomous shuttle service will connect the Torre del Oro and the Seville Aquarium, with extensions to the cruise terminal on specific days. Featuring Level 4 autonomy and V2X-enabled connectivity, the service aims to highlight the future of urban transport systems.